WebApr 13, 2024 · Lipofectamine 2000, Tetro cDNA synthesis kit and QuickExtract DNA extraction were ordered from Invitrogen (USA), Bioline (Germany), and Lucigen (USA), respectively. Primary and secondary antibodies, and fluorescence-tagged secondary antibodies were purchased from Santa Cruz Biotechnology (USA). Midorigreen was … WebJan 6, 2024 · Subsequently, the Tetro cDNA Synthesis Kit was used to convert all RNA samples to cDNA in a 96-well plate using T 100™ Thermal Cycler (Bio-Rad). The qPCR reactions were started by adding 10 ng cDNA to 5 μl SensiFAST™ SYBR and 500 nM forward and reverse primers (Table 3) in a 384-well plate and in duplicates.
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WebJan 31, 2013 · cDNA synthesis was performed with Tetro Reverse Trancriptase kit (Bioline Ltd.) according to the manufacturer’s recommendation. Quantitative PCR was performed using real-time PCR (ABI PRISM 7900, Applied Biosystems). ... reverse transcription reaction using the designed miRNA specific stem loop-RT primer and … Web…the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio,… how many votes to propose an amendment
Tetro cDNA Synthesis Kit - Bioline
http://www.biosave.com/products/tetro-cdna-synthesis-kit-bio-65043 WebTetro cDNA Synthesis Kit (Bioline Reagents) RNaseZap® RNase Decontamination (Ambion®) DEPC-treated water (Ambion®) DNA LoBind 1.5 mL Tubes, PCR clean (Eppendorf) ART® self-sealing barrier pipette tips (Sigma-Aldrich) Eppendorf Mastercycler® PCR Cycler (Eppendorf) StepOnePlus™ Real-Time PCR (Applied Biosystems®) WebAug 5, 2016 · Bioline's Tetro cDNA synthesis kit was used to synthesis first strand cDNA using Salmonella enterica total RNA to quantify gene expression. A total of 1 microgram … how many votes to override filibuster